| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
A single regulatory gene is sufficient to alter Vibrio aestuarianus pathogenicity in oysters
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| Autores: |
Goudenege, David
Travers, Marie-agnes
Lemire, Astrid
Petton, Bruno
Haffner, Philippe
Labreuche, Yannick
Tourbiez, Delphine
Mangenot, Sophie
Calteau, Alexandra
Mazel, Didier
Nicolas, Jean-louis
Jacq, Annick
Le Roux, Frederique
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| Data: |
2015-11
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| Ano: |
2015
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| Resumo: |
Oyster diseases caused by pathogenic vibrios pose a major challenge to the sustainability of oyster farming. In France since 2012 a disease affecting specifically adult oysters has been associated with the presence of Vibrio aestuarianus. Here, by combining genome comparison, phylogenetic analyses and high throughput infections of strains isolated before or during the recent outbreaks, we show that virulent strains cluster into two V. aestuarianus lineages independently of the sampling dates. The bacterial lethal dose was not different between strains isolated before or after 2012. Hence the emergence of a new highly virulent clonal strain is unlikely. Each lineage comprises nearly identical strains, the majority of them being virulent, suggesting that within these phylogenetically coherent virulent lineages a few strains has lost their pathogenicity. Comparative genomics allowed the identification of a single frameshift in a non-virulent strain. This mutation affects the varS gene that codes for a signal transduction histidine-protein kinase. Genetic analyses confirmed that varS is necessary for infection of oysters and for a secreted metalloprotease expression. For the first time in a Vibrio species we show here that VarS is a key factor of pathogenicity
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00226/33685/32530.pdf
DOI:10.1111/1462-2920.12699
https://archimer.ifremer.fr/doc/00226/33685/
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| Editor: |
Wiley-blackwell
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| Formato: |
application/pdf
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| Fonte: |
Environmental Microbiology (1462-2912) (Wiley-blackwell), 2015-11 , Vol. 17 , N. 11 , P. 4189-4199
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| Direitos: |
2014 Society for Applied Microbiology and John Wiley & Sons Ltd
info:eu-repo/semantics/openAccess
restricted use
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